描述
Dulbeccos Modified Eagle Medium
:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of Gibco DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the
media selector tool.
This DMEM / F-12 is modified as follows:
| With |
Without |
| L-glutamine |
HEPES |
The complete formulation is available.
Gibco DMEM / F-12 is a 1:1 mixture of DMEM and Hams F-12. This formulation combines DMEMs high concentrations of glucose, amino acids and vitamins with F-12s wide variety of components.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco DMEM / F-12 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco DMEM / F-12 product made in our Scotland facility (11320-074). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system (2.438 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
详细说明
常用规格
| Form: |
Liquid
|
| Volume: |
500 ml
|
| Glucose: |
High Glucose
|
| Glutamine: |
L-Glutamine
|
| HEPES Buffer: |
No HEPES
|
| Product Size: |
500 mL
|
| Phenol Red Indicator: |
Phenol Red
|
| Serum Supplementation: |
Standard Serum Supplementation
|
| Sodium Pyruvate Additive: |
Sodium Pyruvate
|
| Green Features: |
Sustainable packaging
|
| Shipping Condition: |
Room Temperature
|
内容和储存
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
